Histone mRNA polyadenylation-mediated inflammation underlies various virus infections and cancers.

The mechanistic understanding of virus infection and inflammation in many diseases is incomplete. Normally, messenger RNA (mRNA) tails of replication-dependent histones (RDH) that safeguard naked nuclear DNAs are protected by a specialized stem-loop instead of polyadenylation. Here, we showed that infection by various RNA viruses (including severe acute respiratory syndrome coronavirus 2) induced aberrant polyadenylation of RDH mRNAs (pARDH) that resulted in inflammation or cellular senescence, based on which we constructed a pARDH inflammation score (pARIS). We further investigated pARIS elevation in various disease conditions, including different types of virus infection, cancer, and cellular senescence. Notably, we found that pARIS was positively correlated with coronavirus disease 2019 severity in specific immune cell types. We also detected a subset of HIV-1 elite controllers characterized by pARDH "flipping" potentially mediated by HuR. Importantly, pARIS was positively associated with transcription of endogenous retrovirus but negatively associated with most immune cell infiltration in tumors of various cancer types. Finally, we identified and experimentally verified two pARIS regulators, ADAR1 and ZKSCAN1, which was first linked to inflammation. The ZKSCAN1 was known as a transcription factor but instead was shown to regulate pARIS as a novel RNA binding protein. Both regulators were upregulated under most infection and inflammation conditions. In conclusion, we unraveled a potential antiviral mechanism underlying various types of virus infections and cancers.

Earth Materials and Environmental Applications 2020

The road performance, dynamic properties, and environment impact tests on mechanical property and environmental safety of an industrial residue waste subgrade material were evaluated. The effect of calcium carbonate on the mechanical properties and microstructure of red clay was studied by adding precipitated calcium carbonate to red clay at ratios of 0%, 5%, 10%, 15%, and 20%, and then, shear tests were conducted. In the study of sorptive removal of color dye safranin O (SO) by fibrous clay minerals and zeolites, it was found that the cation exchange capacity (CEC) of the minerals played a key role in SO+ removal and the sorbed SO+ cations were limited to the external surfaces of the minerals due to limited channel size of the fibrous minerals.